Gap Detection Methods for Assessing Salicylate-Induced Tinnitus and Hyperacusis in Rats Purpose A variety of options for behavioral assessment of tinnitus in laboratory animals are available to researchers today. These options are briefly reviewed, followed by data suggesting that gap detection procedures might be used to efficiently measure acute, salicylate-induced tinnitus and possibly hyperacusis in rats. Method Fischer Brown ... Supplement Article
Supplement Article  |   December 01, 2008
Gap Detection Methods for Assessing Salicylate-Induced Tinnitus and Hyperacusis in Rats
 
Author Affiliations & Notes
  • Jeremy G. Turner
    Southern Illinois University School of Medicine, Springfield, and Illinois College, Jacksonville
  • Jennifer Parrish
    Southern Illinois University School of Medicine
  • Disclosure Statement
    Disclosure Statement×
    The first author and M. Kinder are coapplicants on a U.S. patent application that uses procedures similar to those described here to measure tinnitus in lab animals and humans.
    The first author and M. Kinder are coapplicants on a U.S. patent application that uses procedures similar to those described here to measure tinnitus in lab animals and humans.×
  • Contact author: Jeremy G. Turner, Department of Surgery/Otolaryngology Head and Neck Surgery, Southern Illinois University School of Medicine, Springfield, IL 62794-9629. E-mail: jturner@siumed.edu.
Article Information
Hearing & Speech Perception / Acoustics / Hearing Disorders / Supplement: Tinnitus
Supplement Article   |   December 01, 2008
Gap Detection Methods for Assessing Salicylate-Induced Tinnitus and Hyperacusis in Rats
American Journal of Audiology, December 2008, Vol. 17, S185-S192. doi:10.1044/1059-0889(2008/08-0006)
History: Received February 8, 2008 , Accepted September 8, 2008
 
American Journal of Audiology, December 2008, Vol. 17, S185-S192. doi:10.1044/1059-0889(2008/08-0006)
History: Received February 8, 2008; Accepted September 8, 2008
Web of Science® Times Cited: 60

Purpose A variety of options for behavioral assessment of tinnitus in laboratory animals are available to researchers today. These options are briefly reviewed, followed by data suggesting that gap detection procedures might be used to efficiently measure acute, salicylate-induced tinnitus and possibly hyperacusis in rats.

Method Fischer Brown Norway rats (n = 10) were given intraperitoneal injections of 350 mg/kg sodium salicylate on 2 consecutive days, and the effects on gap detection were observed across 9 different frequency bands. Pretest, posttest, and washout data were collected. An additional 4 rats were each given 4 different doses of sodium salicylate (0, 150, 250, and 300 mg/kg), and gap detection and prepulse inhibition were measured.

Results Significant gap detection deficits were observed from pre- to posttest that were consistent with tinnitus. Consistent gap detection deficits were found using broadband noise backgrounds, while significant improvements in responding to frequency-specific test bands were found. Similar effects were repeated in the dose response portion of the study.

Conclusions Gap detection procedures efficiently measured salicylate-induced changes in behavior that were consistent with the presence of tinnitus. In addition, the reliable, stronger responses at many frequencies after salicylate injections suggest the possibility of measuring a hyperacusis-like phenomenon using these methods.

Acknowledgments
Supported in part by National Institutes of Health Grant DC008357 to the first author. Thanks to Dr. Donald Caspary, Dr. Larry Hughes, and Dr. Thomas Brozoski for discussions about this article. Thanks to Michael Kinder and Kinder Scientific for donation of the behavioral equipment used in the current study in honor of the late Dorothy Kinder (Walker).
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